I assume here that the L3 switch is doing routing.
I did not work on the 4500. But generally, as FlipRich said, an extended access list will do well. Say for example you have VLAN1 as 192.168.1.x and VLAN2 as 192.168.2.x. To block traffic from VLAN1 to VLAN2 you would first define an ACL:
[code:1]access-list 100 deny ip 192.168.1.0 0.0.0.255 192.168.2.0 0.0.0.255
access-list 100 permit ip any any[/code:1]
This defines it but doesn't apply it. Now we will apply on Vlan1 interface:
ip access-group 100 in[/code:1]
This will stop the traffic as soon as it enters Vlan1 interface so there is no overhead of routing occurring. The ACL can be defined in other ways, this is one way.